Parameters
CircPrime parameter |
On site |
Parameter description |
|---|---|---|
CircRNA BED file |
CircRNA coordinates |
Input form or upload BED file |
Reference Genome |
Input form |
Currently available “Automatic detection” only |
Shift range left |
shiftA |
Default 150 nucleotides |
Shift range right |
shiftB |
Default 150 nucleotides |
Optimum length of a primer |
PRIMER_OPT_SIZE |
Default 19 nucleotides |
Minimum acceptable length of a primer |
PRIMER_MIN_SIZE |
Default 15 nucleotides |
Maximum acceptable length of a primer |
PRIMER_MAX_SIZE |
Default 20 nucleotides |
PCR product size |
PRIMER_PRODUCT_SIZE_RANGE |
Default 150-200 nucleotides |
Optimum melting temperature for a primer |
PRIMER_OPT_TM |
in Celsius |
Minimum acceptable melting temperature for a primer |
PRIMER_MIN_TM |
in Celsius |
Maximum acceptable melting temperature for a primer |
PRIMER_MAX_TM |
in Celsius |